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Exam 10: Recombinant DNA Technology

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Question 1

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Genetic testing is typically done with blood samples,yet red blood cells (erythrocytes)are anucleate (lack nuclei)and are thus devoid of DNA.How,then,are these tests done?

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Other blood cells,in particula...

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Briefly list the basic steps involved in constructing a DNA fingerprint for a paternity case.

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Cut the DNA of each sample (child,mother...

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How do genetic tests differ from diagnostic tests for genetic disease?

Molecular DNA testing is used to test for aneuploids.

RFLPs arise due to

A potentially serious problem with Taq polymerase in PCR is its lack of proofreading ability.Briefly explain why this can be a problem and at which stages during the PCR process this is most and least problematic.

The Ti plasmid is a natural plasmid in the soil bacterium Agrobacterium tumefaciens.

PCR has revolutionized the criminal justice system through DNA fingerprinting.For a given fragment of DNA,showing that a suspect's fingerprint differs from that found at a crime scene immediately excludes him or her from suspicion,yet finding a match does not mean that guilt can be automatically concluded.Why?

Antisense mRNA can be used to regulate the expression of genes.Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-Savr^TM tomato.

Vectors capable of entering two or more different host organisms are known as

Please select the best match for each term.

Two STR loci were determined to have the following allele frequencies in the general population: $\begin{array} { l l l } & \text { STR A } & \text { STR B } \\\text { Alleles: } & \text { 1A: } 0.1 & \text { 1B: } 0.4 \\& 2 \mathrm {~A} : 0.55 & 2 \mathrm {~B} : 0.4 \\& 3 \mathrm {~A} : 0.25 & \text { 3B: } 0.2 \\& 4 \mathrm {~A} : 0.1 &\end{array}$ A suspect in a criminal trial was genetically tested for these loci and was found to have the genotype (1A,1A)for STR A and (2B,2B)for STR B-homozygous for both loci.What is the probability that a randomly sampled person from this population would also have this genotype?

Which of the following is used to screen newborns for the genetic disease PKU?

A couple with STR genotypes (50,75)and (50,100)(where the allele numbers denote repeat length)have several children.What are the possible genotypes of their children for this STR locus?

A(n) ________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

DNase is used to treat hemophilia.

Rapid detection of SNPs,which are allelic variants,can be accomplished with DNA microarrays.If a microarray is set up for a set of genes,including all variants,outline a general procedure for quickly assaying the genotype of multiple individuals for that set of genes.

Humulin is insulin created by recombinant DNA technology.

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by